Genome edited LOW-seed, HIGH-leaf glucosinolate Indian oilseed mustard

Canola-quality mustard with reduced seed glucosinolate has been developed using CRISPR/Cas9-based editing of 10 glucosinolate transporter (GTR) genes. These transgene-free lines combine nutritional quality with enhanced defence by maintaining high glucosinolates in leaves. Currently, two promising LOW-seed, HIGH-leaf glucosinolate lines are under evaluation in 16 ICAR-coordinated AICRP trial locations.